Coding

Part:BBa_K2978100:Design

Designed by: Jose Pablo Delgado Navarro   Group: iGEM19_Costa_Rica   (2019-10-15)


Auto Inducer Protein for QS in Clostridioides difficile


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 499
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1696


Design Notes

Under a pLac promoter. Therefore needs to be induced with [http://openwetware.org/wiki/IPTG IPTG (Isopropylthiogalactoside)].


Source

Synthesized de novo with proteins codon optimized for E. coli.

References